Safety Manual and Standard Operating Procedures
¶¶Òõ̽̽'s Biosafety Level 3 and Animal Biosafety Level 3 facilities
January 2022
SECTION 1: RESPONSIBILITIES
1.1   PRINCIPAL INVESTIGATOR
1.2  RESEARCH PERSONNELÂ
1.3  ¶¶Òõ̽̽ BIOSAFETY OFFICEÂ
1.4  ¶¶Òõ̽̽ BIOLOGICAL SAFETY COMMITTEE (IBC)
1.5 ¶¶Òõ̽̽ COLLEGE OF MEDICINE & VERMONT DEPARTMENT OF HEALTH (VDH)
1.6 OFFICE OF ANIMAL CARE MANAGEMENT (OACM)
SECTION 2: MEDICAL SURVEILLANCE PROGRAM
2.1  MEDICAL SURVEILLANCEÂ
2.2Â IMMUNIZATIONS
2.3   MEDICAL RESTRICTIONS
2.4   POST-EXPOSURE EVALUATION AND FOLLOW-UP
SECTION 3: LABORATORY PROCEDURES
3.1 ENTRY/EXIT PROCEDURES
3.2 PPE DONNING/DOFFING PROCEDURESÂ
3.3 WORK PRACTICESÂ
3.4 GENERAL A/BSL-3 LABORATORY PRACTICES & PROCEDURESÂ
3.5 [vacated]
3.6  PERSONAL PROTECTIVE EQUIPMENT (PPE)Â
3.7  HANDWASHINGÂ
3.8  PROCEDURES FOR CENTRIFUGATIONÂ
3.9  BIOLOGICAL SAFETY CABINET (BSC) PROTOCOLÂ
3.10   WASTE HANDLING AND DISPOSAL PROCEDURES
3.11  EQUIPMENT REPAIRS, SERVICE, OR DISPOSAL
3.12Â SEMI-ANNUAL SHUTDOWNS
3.13   PROCEDURE FOR LAUNDERING LABORATORY CLOTHING (SCRUBS)
3.14   TRANSPORT OF POTENTIALLY INFECTIOUS MATERIALS BETWEEN A/BSL-3 LABSÂ
3.15Â TRANSPORT OF INACTIVATED MATERIALS TO A/BSL-2 LABS
3.16Â INACTIVATION OF BIOLOGICAL SAMPLES
SECTION 4: SPILL RESPONSE PROCEDURES
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4.1  SPILLS IN BSCÂ
4.2  SPILLS IN THE LABORATORYÂ
4.3  COMPOSITION OF A/BSL-3 SPILL KITÂ
4.4 SPILLS IN INCUBATOR
4.5 SPILLS IN CENTRIFUGE
4.6 CHEMICAL SPILL PROCEDURES
4.7 DECONTAMINATION OF AN A/BSL-3 SPILL
SECTION 5: EMERGENCY RESPONSE PROCEDURES
5.1 EMERGENCY PROCEDURES FOR EXPOSURE INCIDENTS
5.2   EMERGENCY PROCEDURES FOR PERSON DOWN/MEDICAL EMERGENCY (NO SPILL INVOLVED)
5.3   EMERGENCY PROCEDURES FOR MEDICAL EMERGENCY INVOLVING A BIOLOGICAL SPILL
5.4 AFTER HOURS EMERGENCIES
5.5 PROCEDURES FOR FIRE ALARM, HVAC FAILURE, BSC FAILURE, OR POWER LOSS
SECTION 6: MAINTENANCE PERSONNEL AND VISITOR PROCEDURES
6.1  VACCINATION REQUIREMENTSÂ
6.2  PROCEDURES FOR ENTERING A/BSL-3 FACILITYÂ
6.3  PERSONAL PROTECTIVE EQUIPMENT (PPE)Â
6.4  RESPONSIBILITIES OF THE A/BSL-3 FACILITY USERSÂ
6.5 RESPONSIBILITIES OF BIOSAFETY STAFF
6.6  RESPONSIBILITIES OF MAINTENANCE PERSONNEL AND VISITORS
SECTION 7: REGISTERING YOUR WORK
7.1  REGISTRATION OF BIOLOGICAL MATERIALSÂ
7.2  REGISTRATION OF RECOMBINANT DNAÂ
7.3 REQUIREMENTS FOR BSL-3 EXPERIMENTATION IN ANIMALSÂ
7.4 RISK ASSESSMENT
SECTION 8: AUTOCLAVE OPERATION
8.1   A/BSL-3 AUTOCLAVE USE
8.2   AUTOCLAVE VALIDATION
SECTION 9: A/BSL-3 PERSONNEL TRAINING
9.1  A/BSL-3 PERSONNEL TRAINING REQUIREMENTS
9.2  MEMORANDUM OF UNDERSTANDING (MOU) AND AGREEMENT FOR PIÂ
9.3  A/BSL-3 USERS MEMORANDUM OF UNDERSTANDING (MOU) AND AGREEMENT
SECTION 10: FORMS
10.1 A/BSL-3 PERSONNEL TRAINING RECORD
10.2Â STANDARD OPERATING PROCEDURES (SOP) TEMPLATE FOR A/BSL-3 RESEARCH LABORATORIESÂ
10.3  A/BSL-3 VISITOR CLEARANCE FORM
10.4Â RESEARCHER EXPERIENCE FORM
SECTION 11: A/BSL-3 CHECKLISTS
11.1  A/BSL-3 LABORATORY INSPECTION CHECKLISTÂ
11.2  A/BSL-3 FACILITY CHECKLISTÂ
11.3   CHECKLIST FOR MAINTENANCE WORK IN BSL-3 FACILITIES
11.4  A/BSL-3 OBSERVATION CHECKLISTÂ
11.5Â SEMI-ANNUAL A/BSL-3 SHUTDOWN CHECKLIST
SECTION 12: GUIDELINES AND REGULATIONS
12.1  CDC/NIH LABORATORY BIOSAFETY LEVEL CRITERIA - BIOSAFETY LEVEL 3Â
12.2  CDC/NIH VERTEBRATE ANIMAL BIOSAFETY LEVEL CRITERIA - ANIMAL BIOSAFETY LEVEL 3 Â
12.3Â CLASSIFICATION OF HUMAN ETIOLOGIC AGENTS